Big Animals

Metabolic profiles as a tool for preventive medicine in dairy and feedlots: total proteins, albumin and globulins, urea, creatinine, cholesterol, non-esterified fatty acids, beta-hydroxybutyrate, triglycerides, liver enzymes (GOT and gamma-GT), calcium, phosphorus and magnesium.
Metabolic profiles field registration form. Download
Sampling Protocol for Metabolic Profiles. Download
Determinations of transcripts by real-time PCR and localization of proteins by immunohistochemistry.


Hormones

Insulin, IGF-1, Leptin, Adiponectin, Ghrelin,
Progesterone, Estradiol 17 beta, Testosterone, Cortisol, Thyroid hormones

Radioimmunoassay Gamma Counter (Berthold LB 2111)

Chemiluminescence (Immulite 1000)




Metabolic profiles, enzymes,
minerals and others

Total proteins, albumin, urea, creatinine, cholesterol,
non-esterified fatty acids, beta-hydroxybutyrate, triglycerides, Aspartate amino transferase,
gamma glutamyltranspeptidase, Calcium, Phosphorus, Magnesium, Zinc, Haptoglobin, Cytokines

Spectrophotometry (Vitalab Selectra 2)

Microplate reader


Immunohistochemistry and image analysis

Receptors for estrogens, progesterone, oxytocin, leptin,
adiponectin (AdipoR1, AdipoR2), melatonin, interferon tau, and type 1 IGF
Cyclooxygenase 2, IGF-1, cell proliferation antigen (PCNA),
Angiopoietin-like 4 iOsteopontine, Mucine 1

Molecular Biology Platform

Real-time PCR (Corbett), HRM channel real-time PCR

Transcripts of large animals

Availability of determination of transcripts of intervening genes
in the metabolism of different tissues (liver, adipose tissue) and
especially in uterine functionality

SNPs

Determination of Leptin SNPs, Growth Hormone, IGF-1.